Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures.
Identifieur interne : 000212 ( Main/Exploration ); précédent : 000211; suivant : 000213Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures.
Auteurs : I M Gerasymenko [Ukraine] ; Y V Sheludko [Ukraine, Allemagne]Source :
- Biotechnology letters [ 1573-6776 ] ; 2017.
Descripteurs français
- KwdFr :
- Agrobacterium (génétique), Arabidopsis (génétique), Basse température (MeSH), Caulimovirus (génétique), Gènes rapporteurs (MeSH), Protéines recombinantes (biosynthèse), Protéines recombinantes (génétique), Protéines à fluorescence verte (biosynthèse), Protéines à fluorescence verte (génétique), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes végétaux (effets des radiations), Tabac (effets des radiations), Tabac (génétique), Tabac (métabolisme), Tobamovirus (génétique), Transformation génétique (MeSH), Végétaux génétiquement modifiés (effets des radiations), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- biosynthèse : Protéines recombinantes, Protéines à fluorescence verte.
- effets des radiations : Régulation de l'expression des gènes végétaux, Tabac, Végétaux génétiquement modifiés.
- génétique : Agrobacterium, Arabidopsis, Caulimovirus, Protéines recombinantes, Protéines à fluorescence verte, Tabac, Tobamovirus, Végétaux génétiquement modifiés.
- métabolisme : Tabac, Végétaux génétiquement modifiés.
- Basse température, Gènes rapporteurs, Régions promotrices (génétique), Transformation génétique.
English descriptors
- KwdEn :
- Agrobacterium (genetics), Arabidopsis (genetics), Caulimovirus (genetics), Cold Temperature (MeSH), Gene Expression Regulation, Plant (radiation effects), Genes, Reporter (MeSH), Green Fluorescent Proteins (biosynthesis), Green Fluorescent Proteins (genetics), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Plants, Genetically Modified (radiation effects), Promoter Regions, Genetic (MeSH), Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Tobacco (genetics), Tobacco (metabolism), Tobacco (radiation effects), Tobamovirus (genetics), Transformation, Genetic (MeSH).
- MESH :
- chemical , biosynthesis : Green Fluorescent Proteins, Recombinant Proteins.
- genetics : Agrobacterium, Arabidopsis, Caulimovirus, Green Fluorescent Proteins, Plants, Genetically Modified, Recombinant Proteins, Tobacco, Tobamovirus.
- metabolism : Plants, Genetically Modified, Tobacco.
- radiation effects : Gene Expression Regulation, Plant, Plants, Genetically Modified, Tobacco.
- Cold Temperature, Genes, Reporter, Promoter Regions, Genetic, Transformation, Genetic.
Abstract
OBJECTIVES
To exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression.
RESULTS
The efficiency of three different 5'-regulatory sequences to achieve transient expression of the GFP-based reporter gene under chilling conditions (6-8 °C since the 3rd day post inoculation) was compared. We studied the upstream sequences of a cold-inducible Arabidopsis thaliana cor15a gene, the core element of 35S CaMV promoter fused to the TMV omega 5'-UTR, and the synthetic promoter including the 35S core sequence and two binding sites for cold-inducible CBF transcription factors (P_DRE::35S). Cultivation of plants transiently expressing reporter gene under control of the synthetic P_DRE::35S promoter under chilling conditions since the 3rd dpi led to the reliably higher reporter accumulation as compared to the other tested regulatory sequences under chilling or greenhouse conditions. Reporter protein fluorescence under chilling conditions using P_DRE::35S reached 160% as compared to the transient expression in the greenhouse. Period of transient expression considerably extended if plants were cultivated at chilling temperature since the 3rd dpi: reporter protein fluorescence reached its maximum at the 20th dpi and was detected in leaves up to the 65th dpi. The enhanced protein accumulation at low temperature was accompanied by the prolonged period of corresponding mRNA accumulation.
CONCLUSION
Transient expression under chilling conditions using synthetic cold-inducible promoter enhances target protein accumulation and may decrease greenhouse heating expenses.
DOI: 10.1007/s10529-017-2336-z
PubMed: 28439740
Affiliations:
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Genetic Engineering NAS of Ukraine, Zabolotnogo str. 148, Kiev, 03143, Ukraine.</nlm:affiliation><country xml:lang="fr">Ukraine</country><wicri:regionArea>Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Zabolotnogo str. 148, Kiev, 03143</wicri:regionArea><wicri:noRegion>03143</wicri:noRegion></affiliation></author><author><name sortKey="Sheludko, Y V" sort="Sheludko, Y V" uniqKey="Sheludko Y" first="Y V" last="Sheludko">Y V Sheludko</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Zabolotnogo str. 148, Kiev, 03143, Ukraine. ysheludko@ukr.net.</nlm:affiliation><country xml:lang="fr">Ukraine</country><wicri:regionArea>Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Zabolotnogo str. 148, Kiev, 03143</wicri:regionArea><wicri:noRegion>03143</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Plant Biotechnology and Metabolic Engineering, Technische Universität 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Reporter (MeSH)</term><term>Green Fluorescent Proteins (biosynthesis)</term><term>Green Fluorescent Proteins (genetics)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Plants, Genetically Modified (radiation effects)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (genetics)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term><term>Tobacco (radiation effects)</term><term>Tobamovirus (genetics)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium (génétique)</term><term>Arabidopsis (génétique)</term><term>Basse température (MeSH)</term><term>Caulimovirus (génétique)</term><term>Gènes rapporteurs (MeSH)</term><term>Protéines recombinantes (biosynthèse)</term><term>Protéines recombinantes (génétique)</term><term>Protéines à fluorescence verte (biosynthèse)</term><term>Protéines à fluorescence verte (génétique)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes végétaux (effets des radiations)</term><term>Tabac (effets des radiations)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Tobamovirus (génétique)</term><term>Transformation génétique (MeSH)</term><term>Végétaux génétiquement modifiés (effets des radiations)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines recombinantes</term><term>Protéines à fluorescence verte</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Régulation de 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modifiés</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cold Temperature</term><term>Genes, Reporter</term><term>Promoter Regions, Genetic</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Basse température</term><term>Gènes rapporteurs</term><term>Régions promotrices (génétique)</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVES</b></p><p>To exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>The efficiency of three different 5'-regulatory sequences to achieve transient expression of the GFP-based reporter gene under chilling conditions (6-8 °C since the 3rd day post inoculation) was compared. We studied the upstream sequences of a cold-inducible Arabidopsis thaliana cor15a gene, the core element of 35S CaMV promoter fused to the TMV omega 5'-UTR, and the synthetic promoter including the 35S core sequence and two binding sites for cold-inducible CBF transcription factors (P_DRE::35S). Cultivation of plants transiently expressing reporter gene under control of the synthetic P_DRE::35S promoter under chilling conditions since the 3rd dpi led to the reliably higher reporter accumulation as compared to the other tested regulatory sequences under chilling or greenhouse conditions. Reporter protein fluorescence under chilling conditions using P_DRE::35S reached 160% as compared to the transient expression in the greenhouse. Period of transient expression considerably extended if plants were cultivated at chilling temperature since the 3rd dpi: reporter protein fluorescence reached its maximum at the 20th dpi and was detected in leaves up to the 65th dpi. The enhanced protein accumulation at low temperature was accompanied by the prolonged period of corresponding mRNA accumulation.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>Transient expression under chilling conditions using synthetic cold-inducible promoter enhances target protein accumulation and may decrease greenhouse heating expenses.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28439740</PMID><DateCompleted><Year>2018</Year><Month>02</Month><Day>23</Day></DateCompleted><DateRevised><Year>2018</Year><Month>02</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-6776</ISSN><JournalIssue CitedMedium="Internet"><Volume>39</Volume><Issue>7</Issue><PubDate><Year>2017</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Biotechnology letters</Title><ISOAbbreviation>Biotechnol Lett</ISOAbbreviation></Journal><ArticleTitle>Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures.</ArticleTitle><Pagination><MedlinePgn>1059-1067</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10529-017-2336-z</ELocationID><Abstract><AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">To exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The efficiency of three different 5'-regulatory sequences to achieve transient expression of the GFP-based reporter gene under chilling conditions (6-8 °C since the 3rd day post inoculation) was compared. We studied the upstream sequences of a cold-inducible Arabidopsis thaliana cor15a gene, the core element of 35S CaMV promoter fused to the TMV omega 5'-UTR, and the synthetic promoter including the 35S core sequence and two binding sites for cold-inducible CBF transcription factors (P_DRE::35S). Cultivation of plants transiently expressing reporter gene under control of the synthetic P_DRE::35S promoter under chilling conditions since the 3rd dpi led to the reliably higher reporter accumulation as compared to the other tested regulatory sequences under chilling or greenhouse conditions. Reporter protein fluorescence under chilling conditions using P_DRE::35S reached 160% as compared to the transient expression in the greenhouse. Period of transient expression considerably extended if plants were cultivated at chilling temperature since the 3rd dpi: reporter protein fluorescence reached its maximum at the 20th dpi and was detected in leaves up to the 65th dpi. The enhanced protein accumulation at low temperature was accompanied by the prolonged period of corresponding mRNA accumulation.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Transient expression under chilling conditions using synthetic cold-inducible promoter enhances target protein accumulation and may decrease greenhouse heating expenses.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gerasymenko</LastName><ForeName>I M</ForeName><Initials>IM</Initials><AffiliationInfo><Affiliation>Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Zabolotnogo str. 148, Kiev, 03143, Ukraine.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sheludko</LastName><ForeName>Y V</ForeName><Initials>YV</Initials><AffiliationInfo><Affiliation>Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Zabolotnogo str. 148, Kiev, 03143, Ukraine. ysheludko@ukr.net.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant Biotechnology and Metabolic Engineering, Technische Universität Darmstadt, Schnittspahnstrasse 4, 64287, Darmstadt, Germany. ysheludko@ukr.net.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>04</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biotechnol Lett</MedlineTA><NlmUniqueID>8008051</NlmUniqueID><ISSNLinking>0141-5492</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060054" MajorTopicYN="N">Agrobacterium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017796" MajorTopicYN="N">Caulimovirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003080" MajorTopicYN="Y">Cold Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000528" MajorTopicYN="Y">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017898" MajorTopicYN="N">Tobamovirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium-mediated transient expression</Keyword><Keyword MajorTopicYN="N">Cold-inducible promoters</Keyword><Keyword MajorTopicYN="N">Lichenase</Keyword><Keyword MajorTopicYN="N">Nicotiana excelsior</Keyword><Keyword MajorTopicYN="N">cor15a</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>02</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>03</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>4</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>2</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>4</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28439740</ArticleId><ArticleId IdType="doi">10.1007/s10529-017-2336-z</ArticleId><ArticleId IdType="pii">10.1007/s10529-017-2336-z</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Ukraine</li></country><region><li>District de Darmstadt</li><li>Hesse (Land)</li></region><settlement><li>Darmstadt</li></settlement></list><tree><country name="Ukraine"><noRegion><name sortKey="Gerasymenko, I M" sort="Gerasymenko, I M" uniqKey="Gerasymenko I" first="I M" last="Gerasymenko">I M Gerasymenko</name></noRegion><name sortKey="Sheludko, Y V" sort="Sheludko, Y V" uniqKey="Sheludko Y" first="Y V" last="Sheludko">Y V Sheludko</name></country><country name="Allemagne"><region name="Hesse (Land)"><name sortKey="Sheludko, Y V" sort="Sheludko, Y V" uniqKey="Sheludko Y" first="Y V" last="Sheludko">Y V Sheludko</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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